A Study On Aging Associated Changes And Development Of...

(C1.1) Preliminary results: Electrophysiological measurements in the intact abdominal ganglia and analysis of electrophysiological changes in identified neurons during aging. We recently described a ganglia preparation to carry out electrophysiological and molecular analysis of the same neurons in the abdominal ganglia. Using this methodology [31], we have carried out intracellular recordings from L7, L11 and R15 neurons (Fig 2). We then focused on aging associated changes in R15 and have analyzed membrane potentials and response to neurotransmitter Acetylcholine (Ach). We compared two different age groups, mature (6-7 months) and old (11-12 months) animals and found that aging did not alter basal properties such as membrane potential or latency and slope of burst firing whereas we observed a decrease in Ach response in old R15 neurons. To gain deeper insight into Ach induced changes in burst firing; we analyzed four parameters of action potential (AP) waveform (amplitude and duratio n of depolarization and repolarization). We observed specific changes in AP waveform [32]. For example, Ach induced changes in duration of depolarization and repolarization in mature R15 was absent on the old R15 (Fig 3) suggesting a decrease in response to Ach during aging [32]. Multiphoton fluorescence imaging of L7MN in intact abdominal ganglia. As an initial step to trace L7MN in the ganglia to study morphological changes during aging, we have microinjected fluorescent dextran (Alexa FluorShow MoreRelatedFunctional Connections Within Neuronal Circuits839 Words   |  4 Pagesunderstanding human aging and aging-associated cognitive decline, molecular and cellular mechanisms underlying aging associated impairments in learning and memory are still poorly understood. In particular, very little is known about whether physiological and molecular changes in identified neurons that are important for learning lead to aging associated decline in long-term memory storage. We approach this problem by delineating the aging associated changes in two well characterized neurons, presynapticRead MoreMolecular Mechanisms Underlying Aging Associated Decline833 Words   |  4 PagesMechanisms Underlying Aging Associated Decline in Memory Storage Cognitive function depends on proper wiring and functional connections within neuronal circuits. Despite advances in understanding human aging and aging-associated cognitive decline, molecular and cellular mechanisms underlying aging associated impairments in learning and memory are still poorly understood. In particular, very little is known about whether physiological and molecular changes in identified neurons that are important forRead MoreQuetiapine Research Papers5266 Words   |  22 Pageseffect of quetiapine on GABAergic neuron is associated with the prevention of anxiety-like behaviors in aging mice after long-term treatment Authors Abstract Previous studies have demonstrated that quetiapine (QTP) may have neuroprotective properties; however, the underlying mechanisms have not been fully elucidated. In this study, we identified a novel mechanism by which QTP increased the synthesis of ATP in astrocytes and protected GABAergic neurons from aging-induced death. In 12-month-old miceRead MoreThe Effect Of Transport Change During Synapse Formation And Long Term Depression1830 Words   |  8 Pagesmitochondrial transport during synapse formation and long-term depression (LTD). We have made now developed a highly robust assay for quantifying kinesin mediated transport in Aplysia sensory neurons (SNs). Our assay consists of time-lapse imaging of fluorescently labeled mitochondria in sensory neurons. We used mitotracker green to visualize mitochondrial transport and have collected quantitative data on mitochondrial transport in isolated SNs. Then we examined whether transport change during synapse formationRead MoreSleep Disturb ances And The Multifactorial Nature Of Insomnia1548 Words   |  7 PagesAge-Related Changes. The previously discussed models and hypotheses are important in understanding the foundation of sleep disturbances and the multifactorial nature of insomnia. However, it is imperative to also acknowledge the age-related changes that effect sleep architecture and sleep patterns in older adults. The medical field has defined individuals over the age of 65 as elderly, and those over the age of 75 as geriatric. Many changes involving how sleep is initiated and maintained occurRead MoreResearch Proposal on Aging Mind and Memory3134 Words   |  13 PagesAging mind and memory Customer Inserts His/Her Name Customer Inserts Grade Course Customer Inserts Tutor’s Name Writer Inserts Date Here (7, 12, 2010) Contents Abstract 3 Introduction 4 Objectives 5 Methods 6 Discussion 7 Conclusion 11 Literature review 13 Appendix 17 Bibliography 18 abstract The research aims at determining the affect of dementia on the lives of individuals and how it impacts on their social interactions. The research will also identifyRead MoreResearch Proposal on Aging Mind and Memory3142 Words   |  13 PagesAging mind and memory Customer Inserts His/Her Name Customer Inserts Grade Course Customer Inserts Tutor’s Name Writer Inserts Date Here (7, 12, 2010) Contents Abstract 3 Introduction 4 Objectives 5 Methods 6 Discussion 7 Conclusion 11 Literature review 13 Appendix 17 Bibliography 18 abstract The research aims at determining the affect of dementia on the lives of individuals and how it impacts on their social interactions. The research will also identify the ways in which agingRead MoreThe Biological and Psychological Basis of Learning and Memory1875 Words   |  8 Pagesthe brain through lifelong learning to achieve longevity and quality of life. The brain is the center of every functioning part in a human body. Every process stems from the brain. The central nervous system is an intricate menagerie of cells, neurons, cords, nerves, and many other parts that act as the computer center for our bodies. Neuroanatomy is the make-up of the central nervous system. The processes by which the brain acts or performs are neuroprocesses. Learning and memory are two similarRead MoreDiabetes, Ethnicity and Genetics2664 Words   |  11 Pageshave a habit of having similar eating and exercise habits. If you have a family history of type 2 diabetes, it may be difficult to figure out whether your diabetes is due to lifestyle factors or genetic susceptibility. Most likely it is due to both. Studies show that it is possible to delay or prevent type 2 diabetes by exercising and losing weight. Type 2 diabetes is the most common form of diabetes, affecting nearly ninety percent of the twenty-six million Americans with diabetes6. Those at highest Read MoreMicroglial and Neurological Disorder2037 Words   |  9 Pagesimmunity as well as taking part in many CNS development and homeostasis maintenance to support brain integrity. Credited to these roles, emerging evidence implicates microglial as key player that executing both beneficial and detrimental effects in various CNS-related neurological disease including neurodegeneration, neoplastic disease as well as neural development disorders. Emerging evidence implicates microglial play critical roles to the CNS development of the brain. Microglial are unique population